Sample information |
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Picture |
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Location | No map location data available. |
Collection date | 08/24/2023 |
Captive / Cultivated? | Wild-caught |
Group | Georgia Southern University |
Observations | Collected with a sweep net off some flowers. 89 degrees and mostly sunny. |
Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
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Extraction kit | |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | Other |
Gel images |
|
Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
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Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | Positive and negative controls worked as expected as well as NTC. Therefore I feel confident in my results. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Lepidoptera was found to be negative for Wolbachia. |