LOGACMF23-MW Lepidoptera

November 9, 2023

Sample information
Picture
Photos by: MaKayla, W
Location No map location data available.
Collection date 08/24/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected with a sweep net off some flowers.

89 degrees and mostly sunny.
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Positive and negative controls worked as expected as well as NTC. Therefore I feel confident in my results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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