Mosquito 1

March 1, 2024

Sample information
Picture
Photos by: Cameron P
Location
Collection date 08/01/2023
Captive / Cultivated? Wild-caught
Group Stevens Point Area Senior High
Observations
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Anopheles

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We ran numerous tests to prove that it was negative.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Anopheles was found to be negative for Wolbachia.
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