Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/01/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Stevens Point Area Senior High |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Culex |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | there was no line what so ever. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Culex was found to be negative for Wolbachia. |
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata
Cricket- Acheta domesticus