Mosquito 2

March 1, 2024

Sample information
Picture
Location
Collection date 08/01/2023
Captive / Cultivated? Wild-caught
Group Stevens Point Area Senior High
Observations

Male(Fuzzy Antenna)

Culex(Brown Body)

Segmented Body

Black Eyes
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Absolutely no band, making me quite confident that there is no Wolbachia present
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be negative for Wolbachia.
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