Wolbachia Project

March 1, 2024

Sample information
Picture
Photos by: Casey M., Jacob F.
Location
Collection date 08/01/2023
Captive / Cultivated? Wild-caught
Group Stevens Point Area Senior High
Observations

Bushy Antena

Brownish Black

Green Eyes
Putative identification Arthropoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

We selected the medium confidence because there was a line saying it had Wolbachia but it wasn’t a very diffident line.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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