Wolbachia Project

Sample information

Photos by: Casey M., Jacob F.
Collection date 08/01/2023
Captive / Cultivated? Wild-caught
Group Stevens Point Area Senior High

Bushy Antena

Brownish Black

Green Eyes

Putative identification Arthropoda


Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes


Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

We selected the medium confidence because there was a line saying it had Wolbachia but it wasn’t a very diffident line.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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