Pillbug 1-4 Gold

September 11, 2023

Sample information
Picture
Photos by: Kenneth X., Frank X.
Location
Collection date 09/26/2020
Captive / Cultivated? Wild-caught
Group Acton-Boxborough Regional High School
Observations

Collection took place in the fall. The area had some grass but not many other plants or trees (it was a school outdoor garden)
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidae was found to be negative for Wolbachia.
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