Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/26/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Acton-Boxborough Regional High School |
| Observations | Collection took place in the fall. The area had some grass but not many other plants or trees (it was a school outdoor garden) |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Armadillidae was found to be negative for Wolbachia. |
Water Boatman