Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/26/2024 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Scientists in Residence |
| Observations | The specimen was cultivated in a lab, and would have been out for about one day by the point of the experiment. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | My specimen behaved uniquely. Its DNA sequence appeared as wolbachia rather than the insect being tested. While we are unsure what may have happened, our current hypothesis is that the insect had an extreme case of wolbachia to the point that its DNA could not be properly isolated from the infection. |
| Wolbachia 16S sequence |
>1_2_ NNNTNNNTTNNNNNNNNNANCTGGAATTATNGGTCGATTATTATCATTGATTATTCGCACTCAGCNAATGCACATTGNTA TACTTATCAATNACTATCAATTGTATAACTTAATGGTTACCGGGCATGCTTTGATAATGNTGTTTTTTATGATAATGCCA GCCCTCATGGGAGGATTTGGTAACTGGTTTGTACCTCTCATGATTGGCGCACCAAATATGGCGTTTCCTCATATGAATAA TTTGAGTTTCTGGTTATTACCGTCATCTTTTATTTTGCTCATTCTCTCANTGTTTATTGAAGAAGGTCCAGGTACAGGTT GGACTTTATATCCCCCTTTANCACACGTAATGTCCCATCCAAGTGCANGAGTTGACATTGCTATACTTGCACTTCATGTT GCTGGTATGTCNTCAATTGTTGGGGCNNNTTTTTTTATTCTTACTATTTTTAACATGCGCACAAAAGGANTGTCATTAAC TANCATGCCTCTGTTTGTTTGGTCTGTCTTGCNAACATCATTTATTTTGATTGTTGCCNTACCNNTCCNNGCCNGTGCTA TAACTATNCTTCNNACNGANTNNAAATANNTCANTTTNTNATNTGANCNTGCAGGTGGTGGNNACNCTGTGTNANTTCTT CATTTATTTTNANNTTCNGGTCANNNNAAAAATTNAAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
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| Summary | The Drosophila melanogaster was found to be postive for Wolbachia. |
Water Boatman