Fruit Fly/Drosophila melanogaste

May 17, 2024

Sample information
Picture
Location
Collection date 03/26/2024
Captive / Cultivated? Captive / Cultivated
Group Scientists in Residence
Observations

The specimen  was cultivated in a lab, and would have been out for about one day by the point of the experiment.
Putative identification Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My specimen behaved uniquely. Its DNA sequence appeared as wolbachia rather than the insect being tested. While we are unsure what may have happened, our current hypothesis is that the insect had an extreme case of wolbachia to the point that its DNA could not be properly isolated from the infection.
Wolbachia 16S sequence
>1_2_ NNNTNNNTTNNNNNNNNNANCTGGAATTATNGGTCGATTATTATCATTGATTATTCGCACTCAGCNAATGCACATTGNTA TACTTATCAATNACTATCAATTGTATAACTTAATGGTTACCGGGCATGCTTTGATAATGNTGTTTTTTATGATAATGCCA GCCCTCATGGGAGGATTTGGTAACTGGTTTGTACCTCTCATGATTGGCGCACCAAATATGGCGTTTCCTCATATGAATAA TTTGAGTTTCTGGTTATTACCGTCATCTTTTATTTTGCTCATTCTCTCANTGTTTATTGAAGAAGGTCCAGGTACAGGTT GGACTTTATATCCCCCTTTANCACACGTAATGTCCCATCCAAGTGCANGAGTTGACATTGCTATACTTGCACTTCATGTT GCTGGTATGTCNTCAATTGTTGGGGCNNNTTTTTTTATTCTTACTATTTTTAACATGCGCACAAAAGGANTGTCATTAAC TANCATGCCTCTGTTTGTTTGGTCTGTCTTGCNAACATCATTTATTTTGATTGTTGCCNTACCNNTCCNNGCCNGTGCTA TAACTATNCTTCNNACNGANTNNAAATANNTCANTTTNTNATNTGANCNTGCAGGTGGTGGNNACNCTGTGTNANTTCTT CATTTATTTTNANNTTCNGGTCANNNNAAAAATTNAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Drosophila melanogaster was found to be postive for Wolbachia.
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