Antlion larvae and Adult

October 23, 2020

Sample information
Picture
Photos by: Elijah D.
Location
Collection date 08/19/2020
Captive / Cultivated? Wild-caught
Group St. John High School USD 350
Observations

The larvae were found in sandy soil in their pits. There were a lot of other pits around. The adult was found in my backyard.
Putative identification Arthropoda Hexapoda Insecta Neuroptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

I stored my arthropods in 90% ethanol for about two months prior to DNA extraction.

DNA extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.

PCR: Mini One Taq polymerase was used.

Gel Electrophoresis: All of the lanes worked except for the DNA ladder and the positive and negative controls. This may be due to a pipetting error. Importantly though my Wolbachia DNA worked.

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The arthropods were not double banded and it was pretty clear that they didn’t match with the Wolbachia DNA. All of the protocols were followed exactly as well.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Neuroptera was found to be negative for Wolbachia.
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