|Captive / Cultivated?||Wild-caught|
|Group||St. John High School USD 350|
The larvae were found in sandy soil in their pits. There were a lot of other pits around. The adult was found in my backyard.
|Putative identification||Arthropoda Hexapoda Insecta Neuroptera|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniPCR|
I stored my arthropods in 90% ethanol for about two months prior to DNA extraction.
DNA extraction: The DNA was extracted from the abdomen. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.
PCR: Mini One Taq polymerase was used.
Gel Electrophoresis: All of the lanes worked except for the DNA ladder and the positive and negative controls. This may be due to a pipetting error. Importantly though my Wolbachia DNA worked.
|Explanation of confidence level||
The arthropods were not double banded and it was pretty clear that they didn’t match with the Wolbachia DNA. All of the protocols were followed exactly as well.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Neuroptera was found to be negative for Wolbachia.|