Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 03/07/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | I don’t have a photo, but i caught the insect in a small forest near my school. I caught it at 8 o’clock in the morning and the temperature was about 3 degrees. It was under a pile of leaves. Only this insect and no others were sighted in this place. |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Trachelipodidae Trachelipus Trachelipus ratzeburgii |
Methods |
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| Extraction kit | Instagene Matrix |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level |
The result is trustworthy, as all work steps were carried out properly and the DNA duplex worked. In YC2 you can see that a band appeared at 438bp. Therefore you can say that YC2 is a Wolbachia carrier. One band appeared at 708bp. This says that YC1 is an arthropod. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
TGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCCTCGAAGCTGTCCCTTCGAATAGGTATGAGGAGCCCC
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
CCWCGCTCAGGATGATTATTCGAACAGAGCTAGGTTCCCCTGGTAGATTAATTGGGGACGACCAGATTTACAATGTTATTGTTACAGCACATGCTTTTGTTATAATTTTCTTTATAGTAATACCTATTATAATTGGAGGTTTTGGTAATTGATTAGTTCCGTTAATATTAGGGGCTCCTGACATAGCATTCCCGCGTATAAATAATATAAGATTTTGGCTTCTCCCACCTTCTTTAGTTCTTCTCCTTAGAAGAGGGTTGATTGAAAGGGGAGTAGGGACTGGCTGAACTGTTTACCCTCCGTTGGCTGGCGGAATTGCTCACAGAGGGGCTTCAGTAGATTTAGGAATTTTTTCTCTTCATCTTGCGGGGGCTTCTTCAATTTTAGGTGCAGTAAATTTTATTACTACTGTAGCTAATATACGTTCTCCTGGTATAAAAATAGATCGGGTGCCTTTATTTGTATGGTCTGTTTTAATTACAGCTGTTTTATTATTACTTTCGTTACCTGTTTTAGCAGGCGCTATTACTATGTTGCTAACTGACCGTAATTTAAATACATCTTTTTTTGATCCTAGCGGGGGAGGAGACCCTATTTTATACCAGCATTTATTTTGATTTTTTGGTCACCTKGAAAGTTTAAAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Trachelipus ratzeburgii was found to be postive for Wolbachia. |
Water Boatman