DEMAASp24-6 Hymenoptera

Sample information

Photos by: Macey C.
Collection date 01/22/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University

To obtain specimens for our Wolbachia project, we took a D-net to the pond on the
northeast side of campus.
• Between 1:05pm and 1:20pm on January 22, 2024, we collected our specimens. It
was approximately 55 degrees and slightly windy outside.
• Dipping the D-net into the pond, we did not find any insects, but found small fish
and a tiny crustacean. We also discovered a few tiny creatures that resembled
roly-poly bugs.
• We relocated to the bank of the pond where we discovered a large mound of dirt.
We agitated the mound with tweezers, causing ants to stream out.
• We lowered our collection jar to the ground and allowed the ants to enter, catching
• The area was quite swampy while we were collecting, but we did note that the dirt
where the ants were collected was very dry

Putative identification Arthropoda Hexapoda Insecta Hymenoptera


Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used


Wolbachia presence No
Confidence level High
Explanation of confidence level

Hymenoptera does not have a high prevalence of Wolbachia locally and we did not observe a band indicating the Wolbachia 16s rRNA gene.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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