Sample information

To obtain specimens for our Wolbachia project, we took a D-net to the pond on the
northeast side of campus.
• Between 1:05pm and 1:20pm on January 22, 2024, we collected our specimens. It
was approximately 55 degrees and slightly windy outside.
• Dipping the D-net into the pond, we did not find any insects, but found small fish
and a tiny crustacean. We also discovered a few tiny creatures that resembled
roly-poly bugs.
• We relocated to the bank of the pond where we discovered a large mound of dirt.
We agitated the mound with tweezers, causing ants to stream out.
• We lowered our collection jar to the ground and allowed the ants to enter, catching
several.
• The area was quite swampy while we were collecting, but we did note that the dirt
where the ants were collected was very dry

Methods

DNA extraction kit of in-house reagents was used

Results

Hymenoptera does not have a high prevalence of Wolbachia locally and we did not observe a band indicating the Wolbachia 16s rRNA gene.