Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/22/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Time of Collection: 1:15 – 1:25 Location of Collection: East pond on Armstrong Campus. Location of Collection: Mound on the edge of the pond. Habitat: Ant nest/hill Method of Collection: Forceps |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | While Wolbachia infection is not highly prevalent among the Hymenoptera order collected data from the specimen and data from the literature and previous students, I am very confident that my insect specimen is infected with Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Water Boatman