DEMAASp24-09 – Hymenoptera

April 8, 2024

Sample information
Picture
Photos by: Pra'Tasia G.
Location
Collection date 01/22/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Time of Collection: 1:15 – 1:25

Location of Collection: East pond on Armstrong Campus.

Location of Collection: Mound on the edge of the pond.

Habitat: Ant nest/hill

Method of Collection: Forceps
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

While Wolbachia infection is not highly prevalent among the Hymenoptera order  collected data from the specimen and data from the literature and previous students, I am very confident that my insect specimen is infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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