LOGADS24-CR Diptera

April 9, 2024

Sample information
Picture
Photos by: Christine R
Location No map location data available.
Collection date 01/25/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
•2:00 pm
•In front of my house
•Grass
•Warm
•Partially cloudy
•Partially sunny
•Tweezers
•Jar
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

low prevalence rate
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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