Sample information
Picture	Photos by: Christine R
Location	No map location data available.
Collection date	01/25/2024
Captive / Cultivated?	Wild-caught
Group	Georgia Southern University
Observations	•2:00 pm •In front of my house •Grass •Warm •Partially cloudy •Partially sunny •Tweezers •Jar
Putative identification	Arthropoda Hexapoda Insecta Diptera
Methods
Extraction kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes	DNA extraction kit of in-house reagents was used.
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	low prevalence rate
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Diptera was found to be negative for Wolbachia.