Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/16/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collection Location: In between Burnett and Gamble Hall Collection Method: A mason jar Collection Habitat: An ant hill with pine straw Weather Conditions: Cloudy
|
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | When we completed the gel electrophoresis activity only one band showed up proving that my insect was negative for Wolbachia. I also had multiple peer-reviewed sources that showed that there was a low chance of ants being infected with Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid