LOG.AD.S24.SD Hymenoptera

April 9, 2024

Sample information
Picture
Photos by: Skyy D.
Location
Collection date 01/16/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection Location: In between Burnett and Gamble Hall

Collection Method: A mason jar

Collection Habitat: An ant hill with pine straw

Weather Conditions: Cloudy

 
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

When we completed the gel electrophoresis activity only one band showed up proving that my insect was negative for Wolbachia. I also had multiple peer-reviewed sources that showed that there was a low chance of ants being infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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