LOGAD-SW24 Hymenoptera

April 9, 2024

Sample information
Picture
Photos by: Sarah W.
Location
Collection date 01/23/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

date of collection: January 23rd, 2024​

time of collection: Approximately 11:50 am​

location of collection: Between Jenkins Hall and the Quad​

environment (habitat) of collection: Tall flower plants​

method of collection: Enclosed flower within glass jar, carefully removed the jar just enough from the flower to twist the lid back on. 
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents were used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

It is uncommon for Hymenoptera to be Wolbachia positive compared to other orders. Our Gel electrophoresis went exactly how it was expected.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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