KELAGSP524-ARH Hymenoptera

April 12, 2024

Sample information
Picture
Photos by: Angeleah H.
Location No map location data available.
Collection date 01/24/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

My collection location was at school, Georgia Southern University. My collection method was a sweep net and the habitat was tall grass. The weather conditions were wet and very swampy.
Putative identification Arthropoda Hexapoda

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

I would’ve thought my insect had Wolbachia, due to it being popular of having Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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