Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 01/24/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | My collection location was at school, Georgia Southern University. My collection method was a sweep net and the habitat was tall grass. The weather conditions were wet and very swampy. |
| Putative identification | Arthropoda Hexapoda |
Methods |
|
| Extraction kit | |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | I would’ve thought my insect had Wolbachia, due to it being popular of having Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hexapoda was found to be negative for Wolbachia. |
Water Boatman