DEMAFSP24-6 Orthoptera

April 10, 2024

Sample information
Picture
Photos by: Jayce H
Location
Collection date 05/29/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found near the edge of the woods by a pond, at 1:15pm, on a damp and rainy day, using a beat stick and sweep net
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am confident because the controls worked and results showed.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be postive for Wolbachia.
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