Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/29/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Found near the edge of the woods by a pond, at 1:15pm, on a damp and rainy day, using a beat stick and sweep net |
| Putative identification | Arthropoda Hexapoda Insecta |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | I am confident because the controls worked and results showed. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Insecta was found to be postive for Wolbachia. |
Water Boatman