KELABSp24-19 Hymenoptera

April 10, 2024

Sample information
Picture
Photos by: Kennedi S.
Location
Collection date 01/30/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Sunny, but cold and wet when collecting insect.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Rear half
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Further research done has shown that is it uncommon for Hymenoptera to be infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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