Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/05/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | Collected by Fall 2023 students |
| Putative identification | Arthropoda Insecta Hemiptera Pentatomidae Halyomorpha Halyomorpha halys |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | |
| DNA stain | |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | The water control had a DNA band indicating contamination, while DNA+ was empty perhaps indicating a mislabeling error. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
AAAATGCTTGACATTTTTTCGTGTGTGCTCCAACCTCCTGATGGGTCAAAAAAAGAGGTATTAAAATTTCGGTCAGTTAATAGTATAGTAATAGCACCTGCTAACACAGGTAAGGATAATAACAGAAGAAGTGCAGTAATTCCTACTGACCACACAAACAATGGGATTCGTTCAGGGGTTATACCTGTTGGTCGTATATTTATAATAGTTGAAATAAAATTTACTGCCCCTAAAATTGATGAAACCCCAGCCAAATGTAAGGAAAAAATAGCTAAATCAACTGATGCTCCTCTATGTGAGATATTACTTGATAAGGGGGGATAGACTGTTCATCCTGTCCCAGCTCCTGATTCTGCTATTCTTCTTATTAATAATAAAGTTAATGAAGGGGGTAATAATCAGAATCTTATATTATTTAATCGTGGGAAGGCTATATCAGGTGCTCCAATTATTAAAGGGACTAATCAATTACCGAATCCTCCAATTATGATTGGTATTACTATAAAGAAAATTATTACAAATGCATGTGCTGTTACAATTACATTATAAATTTGATCATTACCAATAAATCTTCCAGGCTGTCCTAATTCGATACGGATAATTAATCTTATAGCTGATCCTACTATTCCAGATCATATTCCGAAGATAAAGTATAGAGTTCCAATATCTTTATGTATTAGGTTGCCCACA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Halyomorpha halys was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid