Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 08/25/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | there were multiple specimens of Ectobius v. |
| Putative identification | |
Methods |
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| Extraction kit | Instagene Matrix |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | TBE |
| DNA stain | Midori 1:20'000 |
| Gel images |
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| Protocol notes | gel image not (no more) available |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | no second amplifiaction has been done with sample KZU-007 |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
GGAACTTCTCTAAGTATACTAATTCGGACTGAATTAAATCAACCAGGATCTTTAATTGGAGATGATCAAATTTATAATGT TATTGTTACAGCTCACGCATTTATTATAATTTTTTTTATAGTTATACCCATTTTAATTGGAGGATTTGGAAATTGATTAG TACCTTTAATATTAGGAGCACCAGATATAGCCTTCCCACGAATAAATAATATAAGATTTTGATTATTACCACCATCCCTA ACACTTCTTCTCATGAGAAGTTTAGTAGAAAGAGGGGCAGGAACAGGATGAACAGTTTACCCTCCCTTAGCTAGAGGTAT TGCACATGCAGGAGCATCTGTTGATTTAGCAATTTTCTCATTACATCTTGCTGGAGTATCTTCTATTCTAGGAGCAGTAA ATTTTATTTCCACAATTATTAATATAAAACCTATCAATATAACTTCAGAACAAATTCCTCTCTTCGTTTGATCTGTAGGT ATTACTGCCTTATTATTATTATTATCTTTACCGGTATTAGCTGGTGCTATTACAATATTATTAACAGATCGCAATCTCAA TACATCATTTTTTGATCCCGCAGGAGGAGGTGATCCCATCCTATATCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Ant
Differential Grasshopper – Melanoplus differentialis
Pill Bug (Armadillidium vulgare) – Draft
Melanoplus Femurrubrum
Grasshopper – Orthoptera