Halictidae (Sweat Bee): 2024-022

May 26, 2024

Sample information
Picture
Photos by: John Gruber
Location
Collection date 11/03/2022
Captive / Cultivated? Wild-caught
Group Friends' Central School
Observations

We achieved Wolbachia positive PCR results using three primer pairs: a LepF primer pair, a Wsp (Wolbachia surface protein) primer pair, and a W-spec (Wolbachia specific) 16S primer pair. The 16S sequence we received from the W-spec primer pair PCR matched the sequence from a 16S Wolbachia PCR in Ethiopia.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Halictidae

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

We received Wolbachia positive PCR results using three primer pairs: a LepF primer pair, a Wsp (Wolbachia surface protein) primer pair, and a W-spec (Wolbachia specific) 16S primer pair. The 16S sequence we received from the W-spec primer pair PCR amplification matched the sequence from a 16S Wolbachia PCR amplification in Ethiopia.
Wolbachia 16S sequence
GNNNNNGNNNNNNGGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTNNNNCGAAGCTA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Halictidae was found to be postive for Wolbachia.
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