Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/03/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Friends' Central School |
| Observations | We achieved Wolbachia positive PCR results using three primer pairs: a LepF primer pair, a Wsp (Wolbachia surface protein) primer pair, and a W-spec (Wolbachia specific) 16S primer pair. The 16S sequence we received from the W-spec primer pair PCR matched the sequence from a 16S Wolbachia PCR in Ethiopia. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Halictidae |
Methods |
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| Extraction kit | |
| DNA extraction location | |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | We received Wolbachia positive PCR results using three primer pairs: a LepF primer pair, a Wsp (Wolbachia surface protein) primer pair, and a W-spec (Wolbachia specific) 16S primer pair. The 16S sequence we received from the W-spec primer pair PCR amplification matched the sequence from a 16S Wolbachia PCR amplification in Ethiopia. |
| Wolbachia 16S sequence |
GNNNNNGNNNNNNGGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTNNNNCGAAGCTA
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
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| Summary | The Halictidae was found to be postive for Wolbachia. |
Water Boatman