Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 08/25/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations | resident of our school’s meadow |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Cicadellidae |
Methods |
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| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Midori 1:20'000 |
| Gel images |
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| Protocol notes | no gel image available |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | amplification had been repeated, twice no trace of Wb |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
GATGCTTAGATTTATTATTCGTATTGAACTTTCACAACCAGGTTCATTTTTGGGGAATGACCAAATTTATAATGTAGTTG TTACTTCTCATGCATTTGTAATGATTTTTTTTATAGTTATGCCTATTATAATTGGCGGGTTTGGAAATTGACTTGTTCCA CTAATATTAGGTGCTCCTGATATAGCATTTCCTCGAATAAATAATATGAGATTTTGGTTATTGCCTCCGTCATTAATTTT ATTATTAATGAGATCAATTGTTGAAATAGGTTCTGGAACTGGTTGAACTGTTTATCCACCCCTATCTTCCAATATTTCCC ACTCTGGTCCTAGAGTAGATTTAACTATTTTTTCTTTACATTTGGCTGGTATTTCATCTATTCTTGGGGCTATTAATTTT ATTTCAACTATTATTAATATGCGAATTCAGGGCATAAATATAGATAAAATACCTTTATTTGTTTGATCAGTTTTTGTTAC GGCTATTCTTTTAATGCTTTCATTACCTGTTTTAGCAGGAGCTATTACTATACTATTAACAGATCGTAATTTAAATACAA CCTTTTTTGACCCTTCAGGTGGAGGGGATCCTATTTTGTATCAACATTTATTTTGATTTTTTG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Cicadellidae was found to be negative for Wolbachia. |
Mosquito
Housefly (Musca domestica Linnaeus)
Asian Tiger Mosquito(ATM2685189)
Southern Wood Field Cricket (SWC1089567)