Porcellio scaber

May 27, 2024

Sample information
Picture
Photos by: Ilayda and Marisa
Location
Collection date 08/25/2023
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

under decaying wood blocks
Putative identification Arthropoda Malacostraca Isopoda Porcellionidae Porcellio

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

sample 44 (last lane) stems from Porcellio scaber

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

successful blastn with our wolb-sequence
Wolbachia 16S sequence Download FASTA    Download AB1
TGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAAGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
NNNNCNGGGGCTNNNNGGNNNNCATTNANTATACTTATNCNGGCNGAGTTNNNNNNNCCCGGAGGGTTGATTGGGAGAGA TCAAATTTATAATGTAATTGTTACTGNNCATGCTTTTGTTATAATTNNNNTTATNNTAATACNNATTATANNNNNGGGGT TTGGTAATTGATTANTTCCTTTAATATTAGGGGCCCCAGATATNNCTTTTCCTCGTATGAATAANATANNNTTTTGATTG CTTCCNCCTTCCCTAATACTGCTTCTTANAANAGGATTAGTAGAANNANGGGTAGGAACAGGGTGGACAGTATATCCNCC TTTGGCANCNNNAATTGCNCATANNGGAGCCTCTGTAGATTTAGGAATTTTTTCMTTACATCTTGCAGGGGCNTCTTCNA TTTTAGGGGCTGTAAATTTTATTACTACNATCATTAATATACNGGCNNNNGGGATAANAATANACCGGGTGCCTTTGTTT GTATGGTCTGTAATAATTACNGCANTNNTTTNNNTTNNNNNNTTNCCTGTTTTGNCAGGGGCTATTACTATACTTCNNAC NNANCNAAATTNANNNNNNTCNTTTTNTNNTCCNGGNGGGGGAGGNNNNCCNATTNNTNNNCNNCNNCTNNTTTGATTTT TTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Porcellio was found to be postive for Wolbachia.
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