Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 05/28/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Pingry School |
| Observations | I began by finding a common fly in the wild. From here I captured the bug and placed it in a tube of ethanol. I then crushed the bug to prepare to perform DNA Extraction. Followed by PCR, Gel Electrophoresis, and PCR Purification. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Muscidae Musca Musca domestica |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | The protocol ran smoothly. All controls produced sensible results and the arthropod samples produced results similar to what is expected. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | I am confident in my results because when I compared my DNA sequencing results to those of other flies on NCBI Blast, the sequences of both bugs were very similar. I also confirmed the structural analysis and verified the PCR Results for my bug. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCNCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCACTCGAAGCTA
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
TGGAGCATGAGCAGGAATAATTGGTACTTCATTAAGAATTTTAATTCGAGCAGAATTAGGACATTCTGGAGCTTTAATTGGTGATGACCAAATTTATAATGTAATTGTAACAGCTCACGCTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGTAATTGATTAGTTCCTCTTATACTAGGAGCTCCTGATATAGCTTTCCCTCGAATAAACAATATAAGATTTTGACTTCTTCCTCCTGCACTAACTTTACTATTAATAAGTACATTAATAGAAAATGGAGCTGGAACAGGATGAACTGTTTATCCTCCCTTATCATCTAATATTGCTCATAGAGGACCTTCTGTAGATTTAACTATTTTTTCTCTTCATTTAGCTGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACAACTATTAATATGCGTTCAACAGGTATTACTTTTGATCGAATACCTTTATTTGTTTGATCAGTATTAATTACAGCTATTCTTTTATTACTTTCTTTACCAGTTCTTGCAGGAGCAATTACTATATTATTAACTGATCGAAATATAAATACTTCCTTTTTTGACCCAGCAGGAGGAGGAGATCCTATTCTATACCAACACTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Musca domestica was found to be postive for Wolbachia. |
Water Boatman