Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 05/28/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Pingry School |
| Observations | Note about photograph: unable to capture a clearer photo, as the spider moved very fast. Spider is the silver outer shell within the jar. Spider was captured in the first outside a large building, on top of a bed of moss.
Leucauge venusta– orchard orbweaver
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| Putative identification | Arthropoda Crustacea Hexanauplia Cyclopoida Cyclopidae |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | Although there was a PCR fragment, we did not get good arthropod sequence results. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | There is a 90.21% alignment to the Wolbachia sequence |
| Wolbachia 16S sequence | Download AB1
TGANTCCCACNCNAGTGNCNANGAANTCGGTTAAGTCCCNCCNCCANNNAACCCTCGTCCTTAGTTACNACAGGTAAAGCTGGGGACTTTNANGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTAANATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAANCTAATCCCTTAAAACCCATCTCAGTTCGGATTGNACTCTGCAACTCGAGTGCATGAAGTTGGAATCGNTNGTAATCGTGGATCANCACGACACGGNNAATACNTTCTCGGGTCNTGTANNNANGNCCNTCNNCCCCTGNGAATTGGTTTCACTCCAAGNNNNCNNTNNTTTGCACTTTCNTCTCCTCTTTTCTNTTTTTTACTCCCTNTNAN
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
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| Summary | The Cyclopidae was found to be postive for Wolbachia. |
Brachycybe rosea
Dermacentor variabilis
Hypnorna sp.
Dermacentor variabilis
Dermacentor variabilis