Leucauge venusta

June 6, 2024

Sample information
Picture
Photos by: Sriya T.
Location
Collection date 05/28/2024
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

Note about photograph: unable to capture a clearer photo, as the spider moved very fast. Spider is the silver outer shell within the jar.

Spider was captured in the first outside a large building, on top of a bed of moss.

 

Leucauge venusta– orchard orbweaver
Putative identification Arthropoda Crustacea Hexanauplia Cyclopoida Cyclopidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Although there was a PCR fragment, we did not get good arthropod sequence results.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

There is a 90.21% alignment to the Wolbachia sequence
Wolbachia 16S sequence Download AB1
TGANTCCCACNCNAGTGNCNANGAANTCGGTTAAGTCCCNCCNCCANNNAACCCTCGTCCTTAGTTACNACAGGTAAAGCTGGGGACTTTNANGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTAANATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAANCTAATCCCTTAAAACCCATCTCAGTTCGGATTGNACTCTGCAACTCGAGTGCATGAAGTTGGAATCGNTNGTAATCGTGGATCANCACGACACGGNNAATACNTTCTCGGGTCNTGTANNNANGNCCNTCNNCCCCTGNGAATTGGTTTCACTCCAAGNNNNCNNTNNTTTGCACTTTCNTCTCCTCTTTTCTNTTTTTTACTCCCTNTNAN
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Cyclopidae was found to be postive for Wolbachia.
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