Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 05/31/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Pingry School |
| Observations | The Camponotus seemed to be attracted to the fallen tree. I believe a colony is most likely nearby due to the high number of Camponotus on the log. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Camponotus Camponotus chromaiodes |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | Insect trapping, DNA extraction, DNA amplification, gel electrophoresis, DNA sequencing and analysis |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The gel contained positive fragments for Wolbachia and arthropod which meant that the Compodus contained both arthropod and Wolbachia DNA. My sequencing was good and when I blasted it, it confirmed both arthropod and Wolbachia. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
NNNNNNNNNNTTNNNNNNGTGNNNCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCACTCGAAGCTA
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
NNNNNNNNNNTCTTTGCTATTTGATCGGGANTAGTTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGGTCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGAGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGACCCTTCGGGAGGGGGAGATCCTATTTTATACCAACATTTATTTTGATTTTNNNNNCNNNAAAANTTAAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Camponotus chromaiodes was found to be postive for Wolbachia. |
Water Boatman