BOXELDER BUG

June 3, 2024

Sample information
Picture
Photos by: Sachi T
Location
Collection date 11/30/2023
Captive / Cultivated? Wild-caught
Group Kohler High School
Observations

I noticed that it was big enough to cut its abdomen to get the DNA, so I caught it in hopes of getting Wolbachia.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Rhopalidae

Methods
Extraction kit tweezers
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Took about 3 class periods ,so 5 and a half hours First image- Wolbachia. ORDER OF SEQUENCE: Dna Ladder, Hoveryfly DNA, BOXELDER BUF DNA, positive arthropod control, negative arthropod control, postive DNA control, Water. Second Image- ORDER OF SEQUENCE:Hoverfly Dna, Boxelder Dna, positive arthropod control, negative arthropod control, postive DNA control, Water, Dna Ladder

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confidence because I did the whole lab without contamination and my AP Biology Teacher confirmed it
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Rhopalidae was found to be negative for Wolbachia.
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