Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/30/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Kohler High School |
| Observations | I noticed that it was big enough to cut its abdomen to get the DNA, so I caught it in hopes of getting Wolbachia. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Rhopalidae |
Methods |
|
| Extraction kit | tweezers |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Took about 3 class periods ,so 5 and a half hours First image- Wolbachia. ORDER OF SEQUENCE: Dna Ladder, Hoveryfly DNA, BOXELDER BUF DNA, positive arthropod control, negative arthropod control, postive DNA control, Water. Second Image- ORDER OF SEQUENCE:Hoverfly Dna, Boxelder Dna, positive arthropod control, negative arthropod control, postive DNA control, Water, Dna Ladder |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am confidence because I did the whole lab without contamination and my AP Biology Teacher confirmed it |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Rhopalidae was found to be negative for Wolbachia. |
Brachycybe rosea
Dermacentor variabilis
Hypnorna sp.
Dermacentor variabilis
Dermacentor variabilis