Sample information |
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Picture |
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Location | |
Collection date | 11/10/2023 |
Captive / Cultivated? | Wild-caught |
Group | Kohler High School |
Observations | It looked like a bee Looked weak when caught, most likely due to cold weather Found on doorbell |
Putative identification | Arthropoda Hexapoda Insecta Diptera Syrphidae |
Methods |
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Extraction kit | tweezers |
DNA extraction location | Gonads dissected |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniOne |
Buffer | TBE |
DNA stain | GelGreen |
Gel images |
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Protocol notes | Took about three class periods (5 and a half hours) Arthropod Image: Well 1: Hoverfly Well 2: Boxelder Bug Well 3: Positive Arthropod control Well 4: Negative Arthropod control Well 5: Positive DNA control Well 6: Water Well: DNA Ladder
Wolbachia Image: Well 1: DNA Ladder Well 2: Hoverfly Well 3: Boxelder bug Well 4: Positive Arthropod Control Well 5: Negative Arthropod Control Well 6: Positive DNA Control Well 7: Water |
Results |
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Wolbachia presence | Yes |
Confidence level | High |
Explanation of confidence level | We did this lab with the best care and followed every step to the T, so I believe our results are definitely true. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Syrphidae was found to be postive for Wolbachia. |