Hoverfly

Sample information
Picture	Photos by: Senibua Sander
Location
Collection date	11/10/2023
Captive / Cultivated?	Wild-caught
Group	Kohler High School
Observations	It looked like a bee Looked weak when caught, most likely due to cold weather Found on doorbell
Putative identification	Arthropoda Hexapoda Insecta Diptera Syrphidae
Methods
Extraction kit	tweezers
DNA extraction location	Gonads dissected
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes	Took about three class periods (5 and a half hours) Arthropod Image: Well 1: Hoverfly Well 2: Boxelder Bug Well 3: Positive Arthropod control Well 4: Negative Arthropod control Well 5: Positive DNA control Well 6: Water Well: DNA Ladder Wolbachia Image: Well 1: DNA Ladder Well 2: Hoverfly Well 3: Boxelder bug Well 4: Positive Arthropod Control Well 5: Negative Arthropod Control Well 6: Positive DNA Control Well 7: Water
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	We did this lab with the best care and followed every step to the T, so I believe our results are definitely true.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Syrphidae was found to be postive for Wolbachia.

The Wolbachia Project Database