Nolan-Wolbachia Project

June 3, 2024

Sample information
Picture
Photos by: Nolan V
Location
Collection date 01/18/2024
Captive / Cultivated? Wild-caught
Group Kohler High School
Observations

Lane 4 Centipede DNA-Wolbachia Positive (Very faint in image)

Land 8 Stink Bug DNA-Wolbachia Positive (Very Faint in Image)
Putative identification Arthropoda

Methods
Extraction kit pcr
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

1-DNA Ladder, 2-DNA Ladder, 3-Water, 4-Centipede, 5-Arthropod Control (-), 6-Arthropod Control (+), 7-DNA Control (+), 8-Stink Bug

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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