Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/18/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Kohler High School |
| Observations | Lane 4 Centipede DNA-Wolbachia Positive (Very faint in image) Land 8 Stink Bug DNA-Wolbachia Positive (Very Faint in Image) |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | pcr |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | 1-DNA Ladder, 2-DNA Ladder, 3-Water, 4-Centipede, 5-Arthropod Control (-), 6-Arthropod Control (+), 7-DNA Control (+), 8-Stink Bug |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Water Boatman