Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 05/28/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Pingry School |
| Observations | Has a stinger, seems to be a type of wasp, lives in a terrestrial habitat |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Chalcididae Conura |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | DNA extraction (Cell Lysis, Purification, Elution), PCR, Gel Electrophoresis, PCR Purification, Sequencing |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | During the gel electrophoresis, my arthropod DNA had issues with cross-contamination and was ruined – we did the gel but simply did not get a good sequencing reaction. In addition, the gel electrophoresis for my arthropod DNA had a very faint line while testing for Wolbachia, so I was scared that my arthropod didn’t have it and the band was a result of contamination from the other wells. However, I ended up getting a great result. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
TTTACGNTNCGAATTCGTTTCNTTAGNGCTNATNNAGNNGGNTTTNCGNTTGACNNCTTTAAANCTNNNANGTNACGTTGCANNGNNTAGCGGTAGCCTGTTAACCAANNNGGTNTCTCNNCGCTGATCAAAAGNTNTTCCNATNNAATCNATCNGNNTAGNNATGACTNTGCGNNCGNGTNCNANAATACACTNTGAGNNATGAGAGCCTNAAGGAACANGATTTTTATCNATACTTGGTATCTCNCNACCN
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Conura was found to be postive for Wolbachia. |
Water Boatman