Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/28/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | earthy forest path |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Myrmica Myrmica scabrinodis |
Methods |
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| Extraction kit | Instagene Matrix |
| DNA extraction location | |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Very confident, we always followed the instruction step-by-step. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
ACGAGCGCAACCCTCATCCTTAGWTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGYCGCGAGGCTAAGCTAATCCCTTAAAGGCCATCTCAGYTCGGATTGTACTCTGCWACTCGAGTGCATGAAGKTGGAATCGCTAGTMATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGYTTCACYTCKWWGCTATSYKTRGCGARSTRKGTATRRGTGCTATTAATTTTATCTCAACRATTA
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
CTTCAATAAGAATAATTATTCGTTTAGAATTAGGATCTTGTAACCCATTAATTAATAATGATCAAATTTATAATTCTCTTGTAACTAGCCATGCCTTTATTATAATTTTTTTTATAGTTATACCATTTATAATTGGGGGGCTTTGGTAATTTTTTAA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Myrmica scabrinodis was found to be postive for Wolbachia. |
Water Boatman