Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/18/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Davies Career and Technical High School |
| Observations | Found under a rock, oval-shaped, trying to get away and hide under leaves. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | My confidence level is medium because my arthropod DNA didn’t show up but my Wolbachia DNA showed up. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Water Boatman