Pill Bug – Wolbachia Project #1

November 14, 2024

Sample information
Picture
Photos by: Lilyana S.
Location
Collection date 09/18/2024
Captive / Cultivated? Wild-caught
Group Davies Career and Technical High School
Observations

Found under a rock, oval-shaped, trying to get away and hide under leaves.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My confidence level is medium because my arthropod DNA didn’t show up but my Wolbachia DNA showed up.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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