sarcophaga carnaria_Dreieck_2

Sample information

Picture
Photos by: Alisija Simic
Location
Collection date 08/24/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

caught on dining table

Putative identification Arthropoda Hexapoda Insecta Diptera

Methods

Extraction kit Instagene Matrix
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

The slots were placed at the wrong place, and there wasn’t enough blue tracing dye in the samples.

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level

On gel image 1 there is a faint band visible of our sample triangle 2 in the 5th slot. On gel image 2 there is also no band  visible of our sample triangle 2 in the 3rd slot. In the 3rd image you can see that our initial experiment didn’t give us good results. (Gel 1 and 2 were the results from the repeated experiment in october and gel 3 ist from our first try in september).

Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence Download FASTA    Download AB1
Summary The Diptera was found to be postive for Wolbachia.
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