Polistes dominula Davidstern 04

November 7, 2024

Sample information
Picture
Photos by: Shubham und Dario Gruppe Davidstern
Location
Collection date 08/25/2024
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

Behind the house, lying on the gras without the ability to fly. I caught it with a glas.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Vespidae Polistinae Polistes

Methods
Extraction kit Instagene Matrix
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Midori 1:20'000
Gel images
Protocol notes

strong faint, in the picture its the 4th lane

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

After analyzing the DNA with the BLAST tool, Polistes dominula the was a near to exact match with a  similarity 99.51% so that was the reason for our confidence. As we compared it with our initial identication of the insect with a book we came to the conclusion, that we had found the exact same insect. So the pictures  match with  the result and the identification of the BLAST confirms it. We are very confident that our specimen is a Polistes dominula.

Picture from Wikipedia as a comparison.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TAAGANTAATAATTCGATTAGAATTAGGAACACCATCTTCAATTATTAATAATGATCAATTTTATAATTCAATTATTACA GCCCATGCTTTAGTAATAATTTTTTTTATAGTTATACCATTTATAATTGGAGGTTTTGGAAATTGATTAATTCCTATAAT ACTTGGAGCCCCTGATATAGCTTTCCCACGAATAAATAATATAAGATTTTGACTTTTACCACCCTCATTATTTTTGCTTA TTATAAGAAACATAATAAAAATAGGAGTAGGAACAGGATGAACTTTATACCCACCTTTATCTTCAAATATTGGACATAAT TCACCTTCTGTTGATTTATCAATTTTTTCTCTTCATATTGCTGGAATTTCATCCATTATAGGAGCTATTAATTTTATTGT AACAATTTTAAATATACATATTAAAACCCATTCATTAAATTTTTTACCTTTATTTACATGATCTGTTTTAATTACAGCAA TTTTACTTTTATTATCTTTACCTGTCTTAGCAGGAGCAATCACTATACTATTAACTGATCGAAATTTAAATACATCTTTT TTTGATCCTACAGGAGGAGGTGANCCAATTTTATTTCAACATCTATTTTGANTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Polistes was found to be negative for Wolbachia.
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