GRE5F24-JT Diptera

November 6, 2024

Sample information
Picture
Photos by: Jordan T.
Location
Collection date 08/28/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Time of Collection: 2:54

Method of Collection: Sweeping Net – Waving a net back and forth around tall patches of grass
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit
DNA extraction location In front of University Building
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident because my controls worked the Wolbachia gene was absent and the insect gene was present.
Wolbachia 16S sequence
16S rRNA gene
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
CO1 gene
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
Report Inappropriate Post