Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/28/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Time of Collection: 2:54 Method of Collection: Sweeping Net – Waving a net back and forth around tall patches of grass |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | In front of University Building |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am confident because my controls worked the Wolbachia gene was absent and the insect gene was present. |
| Wolbachia 16S sequence |
16S rRNA gene
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
CO1 gene
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Diptera was found to be negative for Wolbachia. |
Water Boatman