Team Matt Wolbachia project results

November 4, 2024

Sample information
Picture
Photos by: Matt H., Hunter S., Vasil B., Zyir V.
Location
Collection date 09/10/2024
Captive / Cultivated? Wild-caught
Group Owego Free Academy
Observations

Moths and grasshoppers alike are large arthropods with thick exoskeletons that should contain large amounts of DNA
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

In gel 2, lanes 4 and 5 had a small hole about half way down the gel, but this did not effect the results in any way

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We are confident that we did the lab correctly because the control lines are all present. Also, whilst doing the procedures we were very careful and followed the steps very closely.
Wolbachia 16S sequence
detected in positive arthropod control and DNA controL
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
detected in all samples except water
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
Report Inappropriate Post