GREAAF24-HH Coleoptera

November 5, 2024

Sample information
Picture
Photos by: Haydan H
Location
Collection date 08/26/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Method and Location- ​

Beating Cloth Method​

Beat bush with cloth placed under it​

Next to the offices outside of science center​

  • ​
  • Time and Conditions- ​

8/26/24​

1:40 pm.​

 Cloudy​
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of the controls work and it was performed with precision and attention to detail.
Wolbachia 16S sequence
Arthropod COI sequence
TTTCCTATTTggtaGATGAGCAGGaataGTAGGAACCTCCCTTAGACTAC TAATTCGaGCAGAACTCGGAAATCCTGGATCTCTGATTGGAGACGACCAA ATTTACAATGTTATTGTTACTGCACATGCTTTCATCATAATTTTTTTCAT GGTAATACCAATTATAATTGGTGGATTCGGAAACTGGCTTGTCCCTCTAA TACTTGGGGCCCCTGACATAGCTTTCCCCCGAATAAATAACATAAGATTT TGGCTTCTTCCTCCTTCTTTAACTTTACTTTTAATGAGAAGATTAGTAGA AAGAGGAGCTGGAACTGGGTGAACAGTATACCCCCCTTTATCTAGAAACA TCGCTCACAGAGGGGCTTCTGTAGATCTAGCTATTTTTAGTTTACACCTA GCTGGAATTTCATCTATTCTTGGAGCTGTAAATTTCATTACTACAGTTAT TAACATACGtTCTACAGGCATAACATTTGACCGAATACCATTATTTGTAT GATCagtagctTTAACaacTCTTTTACTACTTCTGTCCCTGCCTGTCCTA GCTGGCGCAATCACGATACTTTTAACTGATCGAAATATTAATACCTCATT CTTTGACCCAGCGGGTGGTGGAGACCCAATTTTATACCAACACTTATTTT GATTTTTTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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