Sample information

caught in a public park, temperature of about 70 degrees Fahrenheit, terrestrial habitat.

Two big grey wings, two antennae, appears to be a moth.

Methods

DNA Extraction:

I made sure to crush the arthropod very well, but there were large chunks of exoskeleton that were difficult to break up. As a result, the sample had to be spun 1-2 extra times in the centrifuge for the steps using the filter.

Gel Electrophoresis, arthropod CO1:

1 – DNA ladder

2 – A4 DNA

3 – A5 DNA

4- A6 DNA

5 – Arthropod positive control

6 – Arthropod negative control

7 –  water

Analysis: all controls showed expected bands, and the sample showed an arthropod CO1 band.

Gel electrophoresis, Wolbachia:

1 – DNA ladder

2 – A4 DNA

3 – A5 DNA (lascoria ambigualis)

4- A6 DNA

5 – Wolbachia positive control

6 – wolbachia negative control

7 –  water

Analysis: all controls showed expected bands, and the sample did not show a wolbachia band.

Results

All of my controls worked as expected. I did not have any problems
with the protocol, and I was not surprised by any bands on the gel.
Therefore, I am confident my arthropod is not infected with
Wolbachia.