Boreal Yellow-headed Soil Centipede

Sample information

Picture
Photos by: Michelle G.
Location
Collection date 11/12/2024
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

The arthropod is small, long, and appears a beige yellow. Compared to the rest of the body, the head is slightly darker (burnt sierra color). It has many legs and a long body shape. It is segmented but does not have a thorax or an abdomen (indicating that it is not an insect). Its antennae are long and segmented. It is likely in its early stages of development (juvenile/nymph form); it is a miniature version of the adult, but shorter (about an inch long while the adults can get up to 4 inches).

Putative identification Arthropoda Chilopoda Geophilomorpha Geophilidae Geophilus Geophilus flavus

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

No visible bands appeared in the prospective centipede wells for both the Wolbachia and the arthropod gel electrophoresis. Since it is not evident that there is enough genetic material to analyze, the results are inconclusive, so this sample was not sent in for sequencing.

Results

Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Results are inconclusive since PCR and gel electrophoresis were unsuccessful. Sequencing was not run, so identification cannot be determined. Therefore, I am not confident about what the species is.

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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