Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | The arthropod is small, long, and appears a beige yellow. Compared to the rest of the body, the head is slightly darker (burnt sierra color). It has many legs and a long body shape. It is segmented but does not have a thorax or an abdomen (indicating that it is not an insect). Its antennae are long and segmented. It is likely in its early stages of development (juvenile/nymph form); it is a miniature version of the adult, but shorter (about an inch long while the adults can get up to 4 inches). |
| Putative identification | Arthropoda Chilopoda Geophilomorpha Geophilidae Geophilus Geophilus flavus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | No visible bands appeared in the prospective centipede wells for both the Wolbachia and the arthropod gel electrophoresis. Since it is not evident that there is enough genetic material to analyze, the results are inconclusive, so this sample was not sent in for sequencing. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Results are inconclusive since PCR and gel electrophoresis were unsuccessful. Sequencing was not run, so identification cannot be determined. Therefore, I am not confident about what the species is. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |


Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid