Sample information

Location description: Found on sidewalk
Season: Summer
Temperature: 60°
Habitat: Urban

Color: yellow, orange, dark red

Six legs, two wings, orange antennae, abdomen tapers to a point

Mandibles: dark red

Methods

Gel Electrophoresis, Arthropod CO1 (Round 1):
Lane 1: DNA Ladder
Lane 2-4: Arthropod DNA (A1-A3)
Lane 5: Positive Control
Lane 6: Negative Control
Lane 7: Water

Gel Electrophoresis, Arthropod CO1 (Round 2):
Lane 1: DNA Ladder
Lane 2-4: Arthropod DNA (A4-A6)
Lane 5: Positive Control
Lane 6: Negative Control
Lane 7: Water

Gel Electrophoresis, Wolbachia 16s (Round 1):
Lane 1: DNA Ladder
Lane 2-4: Wolbachia DNA (A1-A3)
Lane 5: Positive Control
Lane 6: Negative Control
Lane 7: Water

Gel Electrophoresis, Wolbachia 16s (Round 2):
Lane 1: DNA Ladder
Lane 2-4: Wolbachia DNA (A4-A6)
Lane 5: Positive Control
Lane 6: Negative Control
Lane 7: Water

Results

I am very confident in the results because the positive and negative Wolbachia controls showed up for Arthropod DNA, which meant that we did the PCR correctly. The water control had no green bands, which meant that there was no cross-contamination.

I am very confident in the results for the Wolbachia DNA because the positive and negative controls for Wolbachia presented as they were supposed to, and we had no Arthropods positive for Wolbachia.