Sample information

In temperate and tropical climates, cicadas reside in trees, shrubs, and subterranean tunnels while they are nymphs. Adults are frequently spotted in the canopy. Wolbachia, a bacterium that controls reproduction through mechanisms like cytoplasmic incompatibility, affects mating patterns and the spread of infected individuals, and is found in many cicada species. Cicadas are well-known for their recurring appearance in enormous “broods,” which frequently occur in cycles of 13 or 17 years and involve billions of individuals emerging at once. Males use a specialized membrane called a tymbal to create loud buzzing calls that can reach 120 dB in order to attract females. Although they only live a brief time as adults, cicadas spend years of their lives underground as nymphs, feasting on tree roots.

Methods

“DNA extraction kit of in-house reagents was used.”

Results

I had strong confidence that my specimen would contain Wolbachia turns out it did not yikes. However , me and my lab partner successfully pushed through these long lab days and was able to get valid lab result which was still an amazing outcome.