Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/01/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Davies Career and Technical High School |
| Observations | Found underneath the bark of a dead log |
| Putative identification | Arthropoda Myriapoda Diplopoda |
Methods |
|
| Extraction kit | Blood and Tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The bug has Wolbachia and the control samples showed up, along with my arthropod having a band with the control. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diplopoda was found to be postive for Wolbachia. |
Water Boatman