Wolbachia Project: Millipede

November 14, 2024

Sample information
Picture
Photos by: Matthew H.
Location
Collection date 11/01/2024
Captive / Cultivated? Wild-caught
Group Davies Career and Technical High School
Observations

Found underneath the bark of a dead log
Putative identification Arthropoda Diplopoda

Methods
Extraction kit Blood and Tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The bug has Wolbachia and the control samples showed up, along with my arthropod having a band with the control.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diplopoda was found to be postive for Wolbachia.
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