Nomophila nearctica A6

November 14, 2024

Sample information
Picture
Photos by: Charlie W
Location
Collection date 09/06/2024
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

I found the arthropod in a small park under a rock during the fall. it was about 75 degrees outside. The arthropod was in a terrestrial habitat. It is gray, and has many legs.

 
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Crambidae Nomophila Nomophila nearctica

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction: I made sure to crush the arthropod really well, the arthropod was small, so no dissection was necessary

Gel Electrophoresis, arthropod CO1:

1 – DNA ladder

2 – A4 DNA

3 – A5 DNA

4- A6 DNA

5 – Arthropod positive control

6 – Arthropod negative control

7 –  water

Analysis: all controls showed expected bands, and the sample showed a arthropod CO1 band.

Gel electrophoresis, Wolbachia:

1 – DNA ladder

2 – A4 DNA

3 – A5 DNA

4- A6 DNA

5 – Wolbachia positive control

6 – wolbachia negative control

7 –  water

Analysis: all controls showed expected bands, and the sample did not show a wolbachia band.

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of my controls worked as expected. I did not have any problems
with the protocol, and I was not surprised by any bands on the gel.
Therefore, I am confident my arthropod is not infected with
Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Nomophila nearctica was found to be negative for Wolbachia.
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