Sample information

Found from a water sample; vibrant yellow in color; immobile after treatment; slight color variations manifested within main body; circular in shape; and likely in larval stage (minuscule size indicates early in development).

Methods

Results

I am fairly confident that the arthropod was Wolbachia positive because I used the entire arthropod’s DNA rather than DNA obtained from the reproductive tissue found in the abdomen, resulting in a higher statistical chance of being able to see Wolbachia; however, the bands were highly distorted in our Wolbachia gel, which can likely be explained by an experimental error that happened during the procedure, causing bands that are not necessarily realistic and concrete, leading to a lack of Wolbachia positive being a safer, more accurate conclusion by the evidence obtained from the experiment. While three of the four controls did not work, the lack of DNA in the water (PCR -) control demonstrates that at least one component of the experiment was accounted for, showing some accuracy in the experimental data.