Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | 3 centimeter long millipede. Has 2 layers of legs on each side of the segmented body, and has one break in the middle, likely due to human injury. Found under a rock curled up, but expanded and began exploring once put into container. |
| Putative identification | Arthropoda Myriapoda Diplopoda Julida Julidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | There was no line in the gel for wolbachia and there was a line for other wolbachia positive segments. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Julidae was found to be negative for Wolbachia. |
Oncopeltus fasciatus
Formica Pallidefulva
Hylephila Phyleus
Thomisus onustus
Halictus Ligatus