Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Hawaii Community College |
| Observations | From my observations the species of mosquito I collected was most likely Aedes albopictus or Asian Tiger Mosquito. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Aedes Aedes albopictus |
Methods |
|
| Extraction kit | pcr |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | lysis |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | My gel had PCR bands around 450 which are in the range for the Wolbachia 16s rRNA. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Aedes albopictus was found to be postive for Wolbachia. |
Water Boatman