Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/12/2024 |
| Captive / Cultivated? | Wild-caught |
| Group | Hawaii Community College |
| Observations | Most likely to be Aedes albopictus or Asian Tiger Mosquito due to the fact that these are day time mosquitos and these were caught in the afternoon when there was still a decent amount of light left. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae Aedes Aedes albopictus |
Methods |
|
| Extraction kit | pcr |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | lysis |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Agarose gel used was created using a 2% Agarose mixture. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | My confidence level is pretty strong due to the fact that in my gel slides the bands that showed for the Wolbachia 16s rRNA were in the same spotted as on the control gel that we were provided with. Also taking note that both bands in my gel slide for Wolbachia 16s rRNA were extremely strong reading. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Aedes albopictus was found to be postive for Wolbachia. |
Water Boatman