Moth

March 9, 2021

Sample information
Picture
Location
Collection date 10/11/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

visible pellet

 

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Wolbachia gel: Wolbachia DNA didn’t work, negative control not loaded (mistake made). Primer Dimers in Wolbachia Gel

Arthropod gel: Arthropod Controls didn’t return band. Water should not have a band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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