Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/11/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | visible pellet
|
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Wolbachia gel: Wolbachia DNA didn’t work, negative control not loaded (mistake made). Primer Dimers in Wolbachia Gel Arthropod gel: Arthropod Controls didn’t return band. Water should not have a band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lepidoptera was found to be negative for Wolbachia. |
Water Boatman