Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/16/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Visible pellet |
| Putative identification | Arthropoda Hexapoda Insecta Phasmida Phasmatidae |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Arthropod gel: Controls didn’t work. Water should not have a band. Wolbachia gel: Wolbachia DNA and controls didn’t work. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Visible pellet Wolbachia DNA and controls didn’t work |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Phasmatidae was found to be negative for Wolbachia. |
Water Boatman