Scarlet Green Leaf Hopper

March 9, 2021

Sample information
Picture
Location
Collection date 10/16/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Visible pellet
Putative identification Arthropoda Hexapoda Insecta Phasmida Phasmatidae

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Arthropod gel: Controls didn’t work. Water should not have a band.

Wolbachia gel: Wolbachia DNA and controls didn’t work.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Visible pellet

Wolbachia DNA and controls didn’t work
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Phasmatidae was found to be negative for Wolbachia.
Report Inappropriate Post