Scarlet Green Leaf Hopper

Sample information

Picture
Location
Collection date 10/16/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Visible pellet

Putative identification Hexapoda Insecta Phasmida Phasmatidae

Methods

Extraction kit Edwards Buffer
DNA extraction location Abdomen
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Arthropod gel: Controls didn’t work. Water should not have a band.

Wolbachia gel: Wolbachia DNA and controls didn’t work.

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Visible pellet

Wolbachia DNA and controls didn’t work

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Phasmatidae was found to be negative for Wolbachia.
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