Pholcus cellar spider

March 31, 2025

Sample information
Picture
Photos by: Jacob B.
Location
Collection date 02/13/2025
Captive / Cultivated? Wild-caught
Group Berkshire Community College
Observations

Spider found indoors in a bathroom.

Link to observation on iNaturalist: https://www.inaturalist.org/observations/261630285
Putative identification Arthropoda Chelicerata Arachnida Araneae Pholcidae Pholcus

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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