Formica glacialis

March 31, 2025

Sample information
Picture
Location
Collection date 09/23/2024
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Caught in the fall on a tree. It is black. It was mainly in the bark.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence Download FASTA    Download AB1
TTGGnnCTTCTATAAGTATAATTATTCGTTTAGAATTAGGTTCGTCTAATTCATTAATCAATAATGATCAAATTTATAAT TCTTTAGTAACTAATCACGCTTTTATTATAATTTTTTTCATAGTAATACCATTTATAATTGGTGGATTTGGAAATTTTCT AATTCCTTTAATACTAGGATCACCAGACATAGCATACCCTCGTATAAATAATATAAGATTTTGACTCTTACCTCCTTCAA TTACTCTTTTACTTTTAAGAAATTTTATTAATGATGGAACAGGAACAGGATGGACTATTTATCCTCCCTTATCCTCTAAT ATTTTTCATAATGGTCCTTCTGTAGACTTAACAATTTTTTCTCTTCATATTGCAGGTATATCCTCAATTTTAGGAGCAAT TAATTTTATTTCAACAATTCTTAATATACATCATAAAAATTTTTCTATTGATAAAATTCCTTTACTCGTATGATCAATTT TAATTACAGCTATCTTACTTCTATTATCCTTACCTGTATTGGCCGGAGCTATTACTATACTATTAACTGATCGAAATTTA AATACTTCATTCTTTGATCCTTCGGGAGGAGGTGATCCAATTCTATATCAACATTTATTTTGAnTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
NGGCTGTCGTNNGCTCGTGTCGTGAGANGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGG TAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTAT GGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCA TCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGA ATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be postive for Wolbachia.
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